A RIBODEPLETION AND TAGGING PROTOCOL TO MULTIPLEX SAMPLES FOR RNA-SEQ BASED VIRUS DETECTION: APPLICATION TO THE CASSAVA VIROME

A ribodepletion and tagging protocol to multiplex samples for RNA-seq based virus detection: application to the cassava virome

Abstract Background Cassava (Manihot esculenta, Crantz), is a staple food and the main source of calories for many populations in Africa, but the plant is beset by several damaging viruses.So far, eight families of virus infecting cassava have been identified; the Geminiviridae (ssDNA viruses responsible for cassava mosaic disease, CMD) and Potyvir

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Separating regular languages with two quantifier alternations

We investigate a famous decision problem in automata theory: separation.Given a class of language C, the separation problem for C takes as input two regular languages and asks whether Water Chamber Air Break there exists a third one which belongs to C, includes the first one and is disjoint from the second.Typically, obtaining an algorithm for sepa

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A Dual Enrichment Strategy Provides Soil- and Digestate-Competent Nitrous Oxide-Respiring Bacteria for Mitigating Climate Forcing in Agriculture

ABSTRACT Manipulating soil metabolism through heavy inoculation with microbes is feasible if organic wastes can be utilized as the substrate for growth and vector as a fertilizer.This, however, requires organisms active in both digestate and soil (generalists).Here, we present a dual enrichment strategy to enrich and isolate such generalists among

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Using Text Mining and Data Visualization Approaches for Investigating Mental Illness from the Perspective of Traditional Chinese Medicine

Background and Objectives.Anxiety and depressive disorders are the most prevalent mental disorders, and due to the COVID-19 pandemic, more people are suffering from anxiety and depressive disorders, and a considerable fraction of COVID-19 survivors have a variety of persistent neuropsychiatric problems after the initial infection.Traditional Chines

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